Fig. 2: NHEJ is not responsible for mutations in the genomes of brc-1 and brd-1 animals.

a Size and junction representation of deletions that were obtained in wild-type and mutant animals (n = 3 for all NHEJ-deficient strains). Deletions without homology are shown in grey, deletions with homology are marked in blue. Increasing homology size is depicted in increasing colour intensity. Deletions with insertions are marked in red. The median deletion sizes are indicated by horizontal lines. b Size and junction representation of TDs that were obtained in wild-type and mutant animals. TDs with homology at the junctions are marked in blue. Increasing homology size is depicted in increasing colour intensity. TDs with insertions are marked in red. The median TD sizes are indicated by horizontal lines. c Quantification of the average rate of deletions per generation in animals of different genotypes. The rate is defined as the number of deletions divided by the number of propagated generations per animal. The rate per strain is represented in blue dots. Two-tailed t-tests were used to determine statistical significance (*P < 0.05, **P < 0.01). d A quantification of the average rate of TDs per generation in animals of different genotypes. The rate is defined as the number of TDs divided by the number of propagated generations per animal. The rate per strain is represented in blue dots. Two-tailed t-tests were used to determine statistical significance (*P < 0.05, **P < 0.01). e A representation of base substitution type and the average rate of base substitutions per generation in animals of different genotypes. The different types of base substitutions are labelled with different colours. The rate is defined as the number of base substitutions divided by the number of propagated generations per animal. The rate per strain is represented in blue dots. Two-tailed t-tests were used to determine statistical significance (*P < 0.05, **P < 0.01). Error bars represent SEM. Wild type: n = 4 of 60 generations, brc-1: n = 6 of 58 generations, cku-80, lig-4, brd-1, brc-1 lig-4 and brd-1 cku-80: n = 3 of 50 generations.