Fig. 5: Molecular and structural analysis of NPY and its precursor.
From: An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles
a Western blot of conditioned media from STC-1 cells stably expressing proNPY using an antibody recognizing pro- and mature NPY. The conditioned media from proNPY-transfected STC-1 cells was concentrated using TCA precipitation, and analyzed with and without neuraminidase treatment (Neu). b Western blot of conditioned media from proNPY-transfected HEK293-6E cells were analyzed with and without neuraminidase treatment (Neu), OpeRATOR treatment, or both in combination. Both western blot experiments in a and b were repeated three times with similar results. c Alpha-helical content of 20 µM NPY or its glycoforms determined by CD spectroscopy (pH 7 at 25 °C) (n = 4 independent measurements for all groups except Tn where n = 3). All error bars represent mean ± S.E.M. Two-tailed one-way ANOVA (Tukey’s post hoc test) was performed in c. ***p < 0.001, **p < 0.01 (all p-values are <0.0001 except c Non-glyc/Tn where p = 0.0018). Source data for a–c are provided as a Source data file.
