Fig. 6: Stability of glycosylated peptide hormones in vitro and in vivo.
From: An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles
a Summary of in vitro degradation of peptide hormones using purified NEP or DPP-IV monitored by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) timecourse assay (See Supplementary Fig. 8 for spectra). Qualitative scoring ++: fully susceptible to degradation, +: reduced susceptibility, −: not susceptible to degradation. b Plasma concentrations of GLP-1 or glycoforms over time after intravenous bolus injection (20 nmol per kg) in anesthetized rats (n = 8 rats per group). GLP-1 was measured by an assay targeting the amidated C-terminus of the molecule measuring total GLP-1 (intact 7–36 amide and metabolites hereof). Half-lives (T1/2) are indicated with 95% confidence intervals. For each glycoform, timepoints represented by n > 4 measurements were included in the analysis (n(GLP-1) = 35, n(GLP-1 T) = 38, n(GLP-1 ST) = 41 measurements in total). Multiple linear regression was performed in b. ns not significant; ***p < 0.001 (all indicated p-values < 0.0001). Source data for b is provided as a Source data file.
