Fig. 4: ZIP-induced GluA1 redistribution in HEK293 cells and AMPAR-EPSCs decrease in the NAc are mediated by NO. | Nature Communications

Fig. 4: ZIP-induced GluA1 redistribution in HEK293 cells and AMPAR-EPSCs decrease in the NAc are mediated by NO.

From: Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors

Fig. 4

a Representative fluorescent images taken from continuous live monitoring of HEK293 cells expressing GFP-GluA1, at 1 and 30 min after bath application of ZIP (5 µM), without (left panels, N = 5) or with L-NAME (50 µM, right panels, N = 5). b AMPAR-EPSCs before and after application of ZIP without (green circles; N = 7, n = 7) or with L-NAME (light blue circles; N = 7 n = 7). Representative EPSC traces taken at the time indicated by the asterisks are shown on the top. Data presented using normalized values ± SEM. c Representative fluorescent images taken from HEK293 cells expressing GFP-GluA1 fixed after 40 min incubation with ZIP (5 μM, left; N = 5), Peptide B (5 μM, middle; N = 6) and Peptide B (5 μM) + L-NAME (50 μM, right; N = 5). d AMPAR-EPSCs recorded before and after application of Peptide B in the presence (light blue circles; N = 5, n = 5) or absence of L-NAME (orange circles; N = 5 n = 5). Data presented using normalized values ± SEM.

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