Fig. 2: Comparison of 3WJ-nBro series for imaging mRNA in vitro and in vivo.
From: A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
a Schematic view of mRNA imaging by 3WJ-nBro series. b Fluorescence changes of 3WJ-nBro series after appending to the 3′ end of AtCLE mRNA. Error bars depict the mean ± s.d. (n = 6) and p values are calculated using an unpaired, two-tailed, Student’s t test. ***p < 0.001. NS, for not significant. c Differential fluorescence decay of 3WJ-nBro series with and without AtCLE mRNA (n = 6). d Representative fluorescence images of 500 E. coli BL21 cells expressing 3WJ-nBro-tagged AtCLE mRNAs after incubation with 10 μM DFHBI-1T. Scale bars, 25 μm. e Fluorescence images of N. benthamiana leaf cells expressing 3WJ-nBro-tagged AtCLE mRNAs after incubation with 10 μM DFHBI-1T. Scale bars, 75 μm. All experiments above were repeated three independent times. Source Data underlying b–e are provided as a Source Data file.
