Table 2 The identification of transgenic Arabidopsis thaliana T2 plants by fluorescence and RT-PCR.

From: A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering

T2 transgenic family

Total plant number

F-P plant number

F-N plant number

N-P plant number

N-N plant number

χ2 value for 3:1

#1

122

93

0

0

29

0.098

#2

114

86

0

0

28

0.012

#3

133

100

0

0

33

0.003

#4

139

105

0

0

34

0.022

#5

116

86

0

0

30

0.046

# 6

142

102

0

0

40

0.761

Total

766

572

0

0

194

0.044

  1. F-P indicates both fluorescent and RT-PCR positive plants; N-N indicates neither fluorescent nor RT-PCR positive plants; F-N indicates fluorescent but RT-PCR negative plants; N-P indicates nonfluorescent but RT-PCR positive plants.
  2. The one-sided χ2 test for 3:1 of F-P plants to N-N plants were conducted with significance level at 0.01. χ21,0.01 = 6.63.
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