Fig. 7: BRD4 loss induces R-loops and DNA damage at a subset of genes. | Nature Communications

Fig. 7: BRD4 loss induces R-loops and DNA damage at a subset of genes.

From: BRD4 prevents the accumulation of R-loops and protects against transcription–replication collision events and DNA damage

Fig. 7

a Diagram showing a subset of BRD4, JMJD6, and CHD4 co-regulated loci that show enrichment of γH2AX using ChIP-Seq following treatment with 500 nM JQ1 for 16 h. b BRD4, γH2AX, and RNAPII ser2 ChIP-Seq plots at the INSIG1 and SRSF2 loci following treatment with DMSO (gray) or 500 nM JQ1 for 16 h (red). In this and subsequent ChIP-Seq figures, layered H3K27Ac ChIP-Seq plots from ENCODE signify actively transcribed loci. c Quantification of relative abundance of DNA:RNA hybrids spanning the INSIG1 and SRSF2 loci following treatment with DMSO (white bars), DMSO + RNase H1 (gray bars), JQ1 (red bars), JQ1 + RNase H1 (blue bars) using DRIP-qPCR (TSS = transcription start site, E = exon, I = intron). Data presented as mean ± SEM (n = 3 separate experiments). Source data are provided as a Source data file.

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