Fig. 2: Zika xrRNA response to pore translocation and stretching.

a Typical snapshots of the initial stages of translocation and stretching processes; the arrows indicate the direction of the force applied inside the pore or at the chain ends. b Temporal trace of the translocated chain fraction from the \({5}^{\prime}\) (blue) and \({3}^{\prime}\) (red) ends for 20 trajectories at the reference temperature T = 300 K and force-ramping rate r = r₀ = 270 pN/μs. The green traces show the longitudinal end-to-end distance, \({R}_{ee}^{\parallel }\) for 20 stretching trajectories at the same values of T and r. The filled curves on the right are the associated height-normalized distributions of the critical forces for translocation and mechanical unfolding, obtained with Gaussian kernel-density estimators. c Typical order of rupture of native contacts during translocation and stretching, see also Supplementary Fig. 2. Secondary elements in a, c are highlighted with the same color code of Fig. 1a.