Fig. 3: Critical translocation forces for different conditions and variants of Zika xrRNA.

a Most probable translocation forces from the \({5}^{\prime}\) and \({3}^{\prime}\) ends at different temperatures, T, and force-ramping rates, r; the estimated errors are smaller than the symbols. For clarity only a subset of all considered (T, r) combinations are shown, see Supplementary Fig. 7. The dashed lines show the Bell–Evans relation of Eq. (1) for T = 300 K with parameters fitted simultaneously on all (T, r) datapoints, see main text. b Box–whisker plots (center line, median ; box limits, upper and lower quartiles) for the translocation forces at T = 300 K and r = r0, for the native (WT) xrRNA (\({5}^{\prime}\) and \({3}^{\prime}\) entries) and for three variants lacking the native contacts in Pk1 and/or Pk2 (\({5}^{\prime}\) entries).