Fig. 4: Structural analysis of the murine DNMT731–1602-DNA complexes. | Nature Communications

Fig. 4: Structural analysis of the murine DNMT731–1602-DNA complexes.

From: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Fig. 4

a Structural overlay of the CCG and ACG complexes (this study) and the GCG complex17 (PDB 4DA4) with the catalytic helix highlighted in expanded view. The disordered regions N-terminal to the catalytic helix are shown as dashed lines in the respective CCG and ACG complexes. The AdoHcy molecule is shown in sphere representation. b Structural overlay of the CCG DNA, ACG DNA and GCG DNA in DNMT1-bound form. The color scheme is the same as in (a). Schematic views of the DNMT1- bound DNAs in the GCG complex (c), adopted from a previous report17, and ACG (d) and CCG (e) complexes. Close-up comparison of the protein-DNA interactions at the CpG sites between the GCG (f), ACG (g) and CCG (h) complexes. The 5-methyl group of the mC6 is shown as green sphere. The hydrogen-bonding and electrostatic interactions are shown as dashed lines in red and black, respectively. Note that in the ACG complex, the side chain of R1237 is non-traceable due to missing electron density.

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