Table 3 Antibodies used for flow cytometry, western blotting and microscopy.

From: Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments

Antibodies

Clone

Source

Identifier/Cat no.

Dilution

CD3 APC Cy7

17A2

BioLegend

100222

1:400

TCRβ BV421

H57-597

BioLegend

109229

1:300

CD122 PE

TM-β1

BioLegend

123209

1:200

Thy1.2 BV 510

53-2.1

BioLegend

140319

1:800

Lag3PerCPefluor 710

C9B7W

eBioscience

46-2231-80

1:300

CD24-BV650

M1/69

BD

563545

1:400

CD8α PECy7

53-6.7

BioLegend

100722

1:200

TCR Vδ4 FITC

GL-2

BD

552143

1:100

TCR Vδ4 PE

GL-2

BioLegend

134905

1:100

CD8β PerCpCy5.5

YTS156.7.7

BioLegend

126610

1:200

TCR Vγ1.1/Cr4 FITC

2.11

BioLegend

141103

1:100

TCR Vγ4 APC

UC3-10A6

BioLegend

137708

1:100

TCRδ BV421

GL3

BioLegend

118119

1:200

TCRδ Pe

GL3

BioLegend

118108

1:800

TCRδ PeCy7

GL3

BioLegend

118124

1:200

CD4 BV 510

RM4-5

BioLegend

100559

1:100

TCR Vδ6.3/2 BV711

8F4H7B7

BD

744476

1:100

CD25-PerCP/Cy5.5

PC61

BioLegend

102030

1:200

TCR Vδ6.3/2-PE

8F4H7B7

Pharmingen

555321

1:300

TCR Vγ7

F2.67

Institut Pasteur, Paris, P. Pereira

N/A

1:400

CD45Rb-FITC

C363.16 A

eBioscience

11-0455-82

1:100

Vγ5-APC

7-17

BioLegend

137506

1:100

TCRδ PerCPeFluor710

GL3

eBioscience

46-5711-82

1:200

TCRδ AF647

GL3

BioLegend

118134

1:200

CD45 PB

HI30

BioLegend

304022

1:300

CD69 PE

H1.2f3

eBioscience

12-0691-93

1:200

CD3 PerCPCy5.5

SK7

BioLegend

344808

1:300

DYDDDDK PeCy7

L5

BioLegend

637324

1:300

HA AF647

16B12

BioLegend

682404

1:200

HIS PE

J095G46

BioLegend

362603

1:100

CD24-BV650

M1/69

BD

563545

1:400

CD62L-BV421

MEL-14

BioLegend

104436

1:300

CD44-Pe-Cy7

IM-7

BioLegend

103030

1:300

CD45 eVolve 605

30-F11

eBioscience

83-0451-42

1:100

TCR Vγ5-PE

536

BioLegend

137504

1:100

Vγ5Vδ1

17D1, Supernatant

Yale, US, R. Tigelaar, J. Lewis

N/A

1:2

TCR-Vg5-FITC

536

BD

553229

1:300

MHC I-A/I-E-AF647

M5/114.15.2

BioLegend

107618

1:500

CD45-eFluor 450

30-F11

eBioscience

48-0451-82

1:200

Skint1

2G2

Monoclonal Antibody core facility (Helmholtz Zentrum Munich)

 

100ul SN /IP

Skint2

3G8

Monoclonal Antibody core facility (Helmholtz Zentrum Munich)

 

1:1000

Flag

M2

Merck

F1804

1:5000

Goat anti rat HRP

 

Thermo Fisher

31470

1:5000

Goat anti mouse HRP

 

Thermo Fisher

31446

1:5000

Flag magnetic beads

M2

Sigma

M8823

25 µl/IP

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