Fig. 2: HDAC8 is in exchange with an alternative state.

a Methyl multiple-quantum CPMG relaxation dispersion profiles for M27 ¹³C^εH₃ of free HDAC8. The curved relaxation dispersion profiles reveal that the major state is in exchange with a low-populated minor state. b Structural representation (PDB: 2v5w¹⁵) of the \(\sqrt {p_{\mathrm{m}}(1 - p_{\mathrm{m}})} |{\mathrm{{\Delta}}}\varpi _{\mathrm{C}}|\) parameters obtained from the CPMG relaxation dispersions, showing the sites affected by the exchange, Supplementary Table 1. c Multiple-quantum relaxation dispersion profiles of M27 ¹³C^εH₃ for TSA-bound HDAC8. In a, c, circles represent experimental data, vertical lines represent the standard derivation (s.d.) and the solid line is the result of a least-squares fit to a two-state model (see text). d Correlation between ¹³C chemical shift differences between free and TSA-bound HDAC8, and |Δϖ| obtained from CPMG relaxation dispersion experiments on TSA-bound HDAC8. Vertical lines represent the standard derivation (s.d.) of the derived |Δϖ| parameters and data points in green are sites located in or near the helix1-loop1-helix2 region.