Fig. 5: CPZ blocks compartment-dependent oncogenic signaling from MT-RTKs. | Nature Communications

Fig. 5: CPZ blocks compartment-dependent oncogenic signaling from MT-RTKs.

From: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V

Fig. 5

a PDI-positive ER fraction was isolated from CALM iKD, CALM SCR, CPZ-treated, and CPZ-untreated MV4-11 cells and subjected to immunoblot analyses. b MV4-11 cells were cultured with CPZ at the indicated concentrations for 18 h. Whole cell lysates or immunoprecipitated proteins were isolated and subjected to immunoblot analyses with the indicated Abs. c LAMP-1-positive protein was immunoprecipitation from the total lysates of CALM iKD, CALM SCR, CPZ-treated, or untreated HMC-1 cells, and subjected to immunoblot analyses using the indicated Abs. d HMC-1 cells were cultured for 24 h with or without CPZ at the indicated concentration, Phosphorylated status of KIT and Akt was assessed by immunoblot analyses on the whole lysates or immunoprecipitated proteins. These densitometry analyses were carried out using Image Quant TL, and data are from two independent experiments.

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