Fig. 1: Enzymatic water-dependent alkyl hydroxylations.
From: ATP-dependent hydroxylation of an unactivated primary carbon with water
ap-Cresol methyl hydroxylase. b Limonene dehydrogenase. c Ethylbenzene dehydrogenase. dp-Cymene dehydrogenase. e Initial steps of anaerobic cholesterol degradation. The enzyme cascade involved in the proposed conversion of 25-OH-CDO (25-OH-cholesta-1,4-diene-3-one) to CDO-26-carboxylate (cholesta-1,4-diene-3-one-26-carboxylate) via a 26-OH-CDO (26-OH-cholesta-1,4-diene-3-one) intermediate was studied in this work.
