Fig. 4: Phylogeny and enrichment of recombinant S26DH1 from S. denitrificans.
From: ATP-dependent hydroxylation of an unactivated primary carbon with water
a Phylogenetic tree of S25DH-like Mo-enzymes of the DMSO reductase family. A. = Aromatoleum; D. = Desulfococcus; α-subunits of CmDH = p-cymene dehydrogenase; EbDH = ethylbenzene dehydrogenase; NarG = nitrate reductase. The previously assigned DH5 is now referred to as S26DH1 (marked in red). b SDS–PAGE of S26DH1 heterologously produced in T. aromatica; the numbers refer to the molecular masses (kDa) of a standard. The band marked with an asterisk was identified as co-purified 6-oxocyclohex-1-ene-1-carboxyl-CoA hydrolase from T. aromatica; the minor band eluting slightly above the 55 kDa is a degradation product of the α5-subunit (Supplementary Table 2). c Cluster of genes encoding the αβγ-subunits and the gene encoding the δ-chaperone of S26DH1 (accession numbers: γβα-subunits, WP_154715926-8; δ-chaperone, WP_154716737). Source data are provided as a Source Data file.
