Fig. 5: Proposed subcellular localisation of initial anaerobic cholesterol degradation steps.
From: ATP-dependent hydroxylation of an unactivated primary carbon with water
For easier presentation, reduction of Δ24 double bond is omitted. The dotted arrows indicate that hydrophobic side chain needs to be bound by S25DH1 and S26DH1. The assignment of S25DH1 and S26DH1 to the periplasm are based on their TAT sequence, that of S25 dehydratase and aldehyde dehydrogenase (ALDH) to the cytoplasm on their cytoplasmic co-substrates. Hydroxylations at C25 and C26 abolish polarity of CDO/DDO and improves access from both sides of the cytoplasmic membrane.
