Fig. 2: Mapping the effect of regulator library genes for enhanced tolerance to isopropanol and isobutanol.
From: Engineering regulatory networks for complex phenotypes in E. coli
a Plasmid barcode‐based mapping of enriched variants across all targeted genes under different conditions. The plots represent the log2 enrichment scores of variants under 30 g/L isopropanol (inner circle) and 8 g/L isobutanol (outer circle). b–e The partial structural models of b Fur (PDB: 4RB1), c Fis (PDB: 5E3O), d RpoE (PDB: 2H27), and e FliA (PDB: 1RP3). The mutated residues for isopropanol experiments are shown in red, the mutated residues for isobutanol experiments are shown in blue, and residues that are enriched in both experiments are shown in purple. f Growth verification of the reconstructed variants compared with nontargeting control under 30 g/L isopropanol or 8 g/L isobutanol. n = 3 for each curve. Error bars show mean value ± SD.
