Fig. 2: Unitary display of ssODN on Cas9 domains enhances HDR in multiple cell types.
From: Engineering designer beta cells with a CRISPR-Cas9 conjugation platform
aHiBiT knockin efficiencies by Cas9-adaptor conjugates compared to unlabeled wild-type Cas9 (wt) when a separate Cas9/ssODN system was used (n = 3 biologically independent experiments except for 204 where n = 2). b–f ssODN display on Cas9 enhances HiBiT knockin efficiency in various cells: b U2OS, c MDA-MB-231, d HEK-293FT, e human-induced pluripotent stem cells, and f primary human neonatal dermal fibroblasts. Unlabeled wild-type Cas9 (wt) and Cas9-adaptor conjugates labeled at the indicated residues were used (n = 3 biologically independent experiments). Source data are provided as a Source Data file.
