Fig. 3: Pre-existing NGFR^hi cells display a stable phenotype.

a Relative MFI as assessed by flow cytometry for parental cell lines after a 24 h co-culture with MART-1 T cells in a 1:8 ratio. After 24 h, T cells were washed off and the medium was refreshed every 3 days. Data shown from three independent replicates. b Relative MFI as assessed by flow cytometry of TR cell lines compared with their parental counterparts over a period of 3 months without any T cell stimulation. c D10 and D10-TR clones were obtained by FACS-based single cell sorting and expansion, and after 2 months they were subjected to MART-1 T cells in a 1:1 ratio. The relative survival compared with parental D10 cells is shown, based on quantification of the corresponding colony formation assays (from two independent replicates). Statistical analysis by Mann–Whitney test by comparing all TR clones to all parental clones; *p < 0.05. d D10 tumors were treated at day 7 with Ctrl or MART-1 T cells in vivo, and at an average tumor volume of 400 mm³ individual mice were re-stratified to sequential BRAF + MEKi treatment (n = 6 per group; dabrafenib 30 mg/kg, trametinib 0.1 mg/kg). Error bars represent S.D. Statistical analysis by Mann–Whitney test on day 7; **p < 0.01. Source data are provided as a Source Data file.