Fig. 3: Treatment of HIV-1 infections in Hu-HSC mice by SECH.

a The SECH regimen includes: (1) latency reversion; (2) induction of cell death; (3) inhibition of autophagy; and (4) blocking of new infections with inhibitors for HIV-1 attachment and integration. b An example of flow cytometry analyses of human cells in the peripheral blood of NSG-SGM3 mice 3 months after reconstitution with human CD34⁺ stem cells. Cell negative for mouse CD45 (mCD45) and positive for human CD45 (hCD45) were gated to analyze CD19⁺ human B cells, CD3⁺CD4⁺, and CD3⁺CD8⁺ human T cells. c Three months after reconstitution with human CD34⁺ stem cells, one set of HIV-1-infected Hu-HSC mice (Supplementary Table 1) were infected with HIV-1 (AD8 strain, 1000 pfu/mouse) intraperitoneally. Ten days after HIV-1 infections, the mice were used for treatments by ART or SECH. d, e RNA from the whole blood (including plasma and cells) was extracted to measure HIV-1 mRNA in mice treated by SECH e or ART f for 40 cycles. The dash line indicates detection limit. f HIV-1 mRNA in the spleen and bone marrow of mice treated by SECH or ART was determined by RT-PCR. Data are presented as mean ± SD (n = 3 technical replicates). ND, not detectable. g Infectious HIV-1 in the spleen and bone marrow of mice treated by SECH or ART was measured by TZA assays. Data are presented as mean ± SD (n = 3 technical replicates). Source data are provided as a Source Data file.