Fig. 2: Cocaine increases population activity of both direct and indirect pathway in DMS.
From: Orbitofrontal-striatal potentiation underlies cocaine-induced hyperactivity
a Representative images of phospho-c-fos staining: D1-tmt (D1-tomato; red) and phospho-c-fos (green). Image area is a 200 µm square of tissue. b Averaged per-mouse (n = 4 mice per condition) c-fos expression after i.p. saline (cSAL) or cocaine injections in dMSNs and iMSNs (RM two-way ANOVA; cell-type main effect: F(1,6) = 3.998, p = 0.0925; drug main effect: F(1,6) = 14.53, p = 0.0088; cell-type Χ drug interaction: F(1,6) = 4.634, p = 0.0748; followed by between-subject post hoc False Discovery Rate method of Benjamini, Krieger and Yekuteli test saline vs cocaine; dMSNs: t(12) = 4.370; iMSN: t(12) = 2.488). c Representative images of GCaMP6s expression in DMS (V ventricle, AC anterior commissure, Cx cortex). Scale bar: 1 mm. d Experimental and brain schematics of fiber photometry experiments with example photometry traces. Scale bar: 30 ms, 5% dF/F. e Norm. frequency of direct pathway calcium events (RM two-way ANOVA by both factors; time main effect: F(59,413) = 1.063, p = 0.3585; drug main effect: F(1,7) = 20.37, p = 0.0028; time Χ drug interaction: F(59,413) = 2.891, p < 0.0001). f Binned norm. frequency (RM two-way ANOVA by both factors; time main effect: F(1,7) =  0.9040, p = 0.3734; drug main effect: F(1,7) = 53.77, p = 0.0002, time Χ drug interaction: F(1,7) = 72.32, p < 0.0001, followed by between-subject Bonferroni post-hoc test Saline vs Cocaine; 11–30: t(7) = 1.539, p = 0.3354; 31–60: t(7) = 8.137). g Norm. frequency of indirect pathway calcium events (RM two-way ANOVA by both factors; time main effect: F(59,295) = 1.118, p = 0.2729; drug main effect: F(1,5) = 21.45, p = 0.0057; time Χ drug interaction: F(59,295) = 2.5, p < 0.0001). h Binned frequency (RM two-way ANOVA by both factors; time main effect: F(1,5) = 9.215, p = 0.0289; drug main effect: F(1,5) = 13.48, p = 0.0144; time Χ drug interaction: F(1,5) = 25.37, p = 0.0040; followed by between-subject Bonferroni post-hoc test Saline vs Cocaine; 11–30: t(5) = 0.9309, p = 0.7893; 31–60: t(5) = 4.583). Data are represented as single points and/or as mean ± SEM. N indicates number of mice.
