Fig. 1: Efficiency and accuracy of cellBC and UMI assignment.
From: High throughput error corrected Nanopore single cell transcriptome sequencing
a CellBC and UMI assignment strategy (Detailed in Supplementary Fig. 2). b Efficiency of cellBC and UMI identification for eight PromethION sequencing runs (n = 8): both poly(A) and adapter, 57 ± 11% (SD) of total reads; cellBC, 68 ± 4% (SD) of reads with identified poly(A) and adapter; UMI, 76 ± 3% (SD) of reads with identified cellBC. Boxes and error bars indicate the means and standard deviations, respectively, for n = 8 flow cells. c UMI sequencing depth (reads/UMI) for the Illumina and Nanopore dataset. d Accuracy of cellBC and UMI assignment. Number of cellBC and UMI assigned reads before and after replacement of cellBC or UMIs with random sequences and after cellBC and UMI identification guided by an unrelated Illumina dataset (24 × 106 reads of the 190 cell sample were scanned). e, f Edit distance distribution of the cellBC and UMI assignment.
