Fig. 3: Nanopore scRNA-seq reveals transcript isoform diversity.
From: High throughput error corrected Nanopore single cell transcriptome sequencing
a–c Clta isoform expression switch during neuronal maturation visualized on the t-SNE plot of Fig. 2d. a Clta-204 (ENSMUST00000107849.9), b Clta-206 (ENSMUST00000170241.7), c Overlay Clta-204/Clta- 206. d Principal Clta splice variants and supporting genome-aligned reads for two UMIs. A read not consistent with the UMI consensus (likely PCR artifact) is labeled with an “*”. e Relative expression of known Ensembl Clta transcript isoforms in different cell types of the brain (clusters of Fig. 2d).
