Fig. 4: Inhibition of autophagy restores mitochondrial content in axons of C. elegans carrying mitochondrial dysfunction.
From: Inhibition of autophagy curtails visual loss in a model of autosomal dominant optic atrophy
a Confocal images of mtGFP (green) and cytoplasmic mCherry (red) in GABA motor neurons of the indicated transgenic nematodes. Bar, 20 μm. b Quantification of mitochondrial content in axons in n = 13 animals from three independent experiments as in (a). ****p < 0.0001 in one-way ANOVA/Tukey’s test. c Confocal images of mtGFP (green) and cytoplasmic mCherry (red) in GABA motor neurons of transgenic nematodes treated as indicated. Upper panels: magnified soma region. PQ, paraquat. Bars, 20 μm. d Quantification of mitochondrial content in axons in n = 25 animals from three independent experiments as in (c). **p = 0.0012 in one-way ANOVA/Tukey’s test. e Confocal images of mtGFP (green) and cytoplasmic mCherry (red) in GABA motor neurons of transgenic nematodes treated as indicated. Bar, 20 μm. f Quantification of mitochondrial content in axons in n = 25 animals from three independent experiments as in (e). ****p < 0.0001 in one-way ANOVA/Tukey’s test. g Confocal images of mtGFP (green) and cytoplasmic mCherry (red) in GABA motor neurons of transgenic nematodes treated as indicated. Bar, 20 μm. h Quantification of mitochondrial content in axons in n = 25 animals from three independent experiments as in (g). ****p < 0.0001 in one-way ANOVA/Tukey’s test. i Confocal images of mtGFP (green) and cytoplasmic mCherry (red) in GABAergic motor neurons of the indicated transgenic nematodes expressing Opa1K301A. Bar, 20 μm. j Quantification of mitochondrial content in axons in n = 25 animals from three independent experiments as in (i). ****p < 0.0001 in one-way ANOVA/Tukey’s test. k Quantification of defecation motor program duration recorded in n = 10 transgenic nematodes from three independent experiments at the indicated days of life. ****p < 0.0001 in one-way ANOVA/Tukey test. In box plots, centre line represents mean, bounds of boxes SEM, whiskers the 10th–90th percentiles; each dot represents an individual nematode form the indicated N of independent experiments. Source data are provided as a Source Data file.
