Fig. 3: Excitation-energy transfer from Wp to Wc.
From: A leap in quantum efficiency through light harvesting in photoreceptor UVR8
a–c RET rate distributions based on QM/MM calculations for each Wp–Wc pair. a W198 as the donor. b W250 as the donor. c W302 as the donor. In each figure, the distribution of the total RET rate to 4Wc is shown in black and the most dominant energy acceptor is underscored. d Simulation of energy-transfer dynamics for 3Wp to 4Wc based on RET rate distributions. For all 3Wp, a fast energy transfer (τtotal1) and a slow energy transfer (τtotal2) are needed to fit the transients. e The 12 possible energy-transfer pathways from 3Wp to 4Wc as calculated with the FRET theory. Each line represents one energy-transfer pathway. Colors of the lines are based on calculated RET time constants. The total RET time constants are shown near each Wp. f Fluorescence dynamics of selected 6Wd + 1Wp at excitation of 310 nm. g Fluorescence dynamics of WT at excitation of 315 nm. h–k Model simulations of typical fluorescence transients at 340 nm for mutants 6Wd + 1Wp + 4Wc(h), 6Wd + 2Wp + 4Wc (i), 6Wd + 3Wp + 4Wc (j), and WT (k). In each figure, the black solid line is the experimental data and the green solid line is the total simulation curve, which is the sum of contributed ones from Wc (red line), Wp (dark yellow line), and Wd (purple line). The simulation method is detailed in Methods and Supplementary Methods. l–o Comparison between directly measured and simulation-constructed spectra for 6Wd + 1Wp + 4Wc (l), 6Wd + 2Wp + 4Wc (m), 6Wd + 3Wp + 4Wc (n), and WT (o). In each panel, the black line is the total emission. The colored symbols represent the spectra of three W groups decomposed from the total emission based on the time integrals of simulation curves (Supplementary Figs. 9–13). Solid lines are directly measured emission spectra of the three W groups.
