Fig. 2: OLs differentiate in a Gsta4-dependent manner.

a Illustration of Dark Agouti (DA) rat over-expressing Gsta4 (DA^Gsta4). b In vivo transcription of Gsta4 across the OL lineage in adult DA^Gsta4 and DA^Wt (n = 4). c GSEA, analyzed using weighted enrichment statistics and ratio of classes for the metric as input parameters, for CNS bulk tissue (cortex, corpus callosum and hippocampus) from adult DA^Gsta4 and DA^Wt (n = 4). d Transcription of Plp1 following 48h differentiation of neonatal DA^Gsta4 or DA^Wt-derived OPCs in the presence or in absence of bFGF (n = 3). e In vitro proliferation of DA^Wt and DA^Gsta4 OPCs for 72h in various concentration of Fgf (n = 4). f Transcription of Mbp and Plp1 following 48 h differentiation of neonatal DA^Gsta4 or DA^Wt-derived OPCs in combination with Gsta4 knockdown with siRNA-Gsta4 or siRNA-Neg as a scrambled sequence (n = 4). g Immunocytochemistry of Plp protein expression following Gsta4 knockdown with siRNA-Gsta4 (n = 10). h Representative images at day 5, scale bar 50 μm. Graphs depict representative results from two (b, d–h) independent experiments. All data are presented as mean and S.D. All statistical analyses were performed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.