Fig. 5: Gsta4 stimulates axonal remyelination through more efficient OL differentiation.

a Corpus callosum sections stained with LFB for evaluation of demyelination, scale-bar 1 mm. b LFB staining following administration of 0.1% LPC (naïve/24h/72h; n = 6, 15d; n = 5). c Representative TEM images of corpus callosum, myelinated axons pseudo-colored in blue, scale-bar 2 μm and 400 nm. d Percentage of myelinated axons in naïve DA^Wt and DA^Gsta4 and during remyelination (n = 3). eg-ratio in naïve DA^Wt and DA^Gsta4 and during remyelination (n = 3). f Correlation between g-ratio and axonal mitochondrial size during homeostasis (left) and during remyelination (right) (n = 30). g Illustration of experimental set-up. h Flow cytometric analysis of total number of PDGFRα⁺ OPCs (green) (n = 5) and O1⁺ and O1⁺ OLs (grey/blue) (n = 5 + 6). i Immunohistochemically validation of CC1⁺ OLs in corpus callosum (n = 5). j Representative corpus callosum images 10 days following LPC, scale-bar 10 μm. k Number of PDGFRα⁺, O4⁺, or O1⁺ double-positive for EdU⁺ 10 days after continuous EdU administration. l Number of CC1⁺ double-positive for EdU⁺ 10 days after EdU administration. All graphs show mean and S.D., apart from i, l showing box and whiskers, indicating values outside 5–95 percentile. All statistical analyses were performed with one-way ANOVA, apart from i, l performed with Students’ two-tailed unpaired t test and f analyzed with two-tailed Pearsons’ r test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.