Fig. 6: Gsta4 in CNS ameliorates EAE and reduce demyelinating lesions.

a EAE was induced through subcutaneous injection of MOG in adjuvant (n = 26 + 17). b Disease duration and cumulative score based on a. c mRNA expression of Fas-associated Faf1 and Dffa in spinal cord O4⁺ OLs at day 30 (dotted line in a). d Levels of MitoSOX in O1⁺ OLs from spinal cord at day 50 from a. e, f Illustration of experimental set-up and behavioral EAE score in lethally irradiated DA^Wt (n = 12) and DA^Gsta4 (n = 16) transplanted with DA^GFP bone marrow. g Disease duration and max score based on f. h, i Percentage of Casp8⁺CC1⁺ of all CC1⁺ (n = 3) and number of Casp8⁺CC1⁺ OLs in spinal cord (n = 3) at day 30 based on representative animals in e. j, k Demyelinated area of total area of dorsal column and number of dorsal column lesions (n = 3) at day 30 based on representative animals in e. l Representative images, scale-bar 100 μm. All data are shown as mean and S.D. apart from b, g showing violin plots and mean. Two-way ANOVA test was used for a, f to analyze differences between groups over-time, graphs are showing mean and S.E.M. Remaining statistical analyses were performed using Student’s two-tailed unpaired t test, apart from disease duration and number of lesions analyzed with Mann–Whitney test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.