Fig. 8: vFB1-3 are distinct cell types secreting VEGF-C.

a Representative images of vFB3 (P2X1⁺Serpina3n⁺PDGFRβ⁺) and vFB1-2 (P2X1^-Serpina3n⁺PDGFRβ⁺) in WT mouse. Similar findings were observed in n = 4 mice from two independent experiments. White and yellow dotted boxes are magnified in the upper right and lower right panels, respectively. Red and yellow arrowheads indicate vFB3 and vFB1-2, respectively. Scale bar, 20 μm. b Representative images of vFB1 (Serpina3n⁺Fosb⁺Shisa3⁺PDGFRβ⁺) and vFB2 (Serpina3n⁺Fosb^-Shisa3⁺PDGFRβ⁺) in WT mouse. Similar findings were observed in n = 4 mice from two independent experiments. White and yellow dotted boxes are magnified in the upper right and lower right panels, respectively, for each image. White and yellow arrowheads indicate vFB1 and vFB2, respectively. Scale bars, 20 μm. c Representative images of Vegfc single-molecule fluorescence in situ hybridization (smFISH) in intestinal villi of tdTomato^rPβC and Lats1/2^iΔ- tdTomato^rPβC mice. Similar findings were observed in n = 4 mice/group from two independent experiments. White dotted boxes are magnified in the right panel. Scale bars, 20 μm. d Representative images of Vegfc smFISH in vFB1-3 of WT and Lats1/2^iΔ- tdTomato^rPβC mice. Similar findings were observed in n = 4 mice/group from two independent experiments. White and yellow dotted boxes are magnified in the upper right and lower right panels, respectively, for each image. Scale bars, 20 μm.