Fig. 4: Mutant core segment and the corresponding wildtype segment show distinct features.
From: CryoEM structure of the low-complexity domain of hnRNPA2 and its conversion to pathogenic amyloid
a The steric zipper structure determined for mutant segment GNYNVF, viewed down the fibril axis. Water molecules within the structures are shown as aqua spheres. The unit cell is shown as a box. b A superimposition of the mutant steric zipper structure of GNYNVF (blue) on the cryoEM mC-hnRNPA2-LCD structure (gray) shows the incompatibility of the mutant structure with the fold of the wildtype fibril. Only the corresponding part of the wildtype structure is shown. The sidechains of Asn287, Tyr288, and Asn289 of the mutant structure experience steric clashes with the sidechains of Asn282, Asp290, and Met304 of the wildtype structure. c Left: Mutant segment GNYNVF shaken after 4 days forms a white precipitate. Right: Wildtype segment GNYNDF shaken after 4 days forms a hydrogel. d Left: The mutant segment GNYNVF (from c) visualized by transmission electron microscopy, shows wide needle crystals. Scale bar: 200 nm; Right: Wildtype segment GNYNDF (from c) visualized by transmission electron microscopy, shows thin fibrils.
