Fig. 4: The RdDM pathway is active in the rice SAM.
From: DNA methylation is reconfigured at the onset of reproduction in rice shoot apical meristem
a Schematic diagram of the canonical RdDM pathway. b Heat map showing the expression patterns of canonical RdDM pathway genes and rice homologs of CMT2 and DDM1 in mature leaf blade (Leaf), vegetative SAM (SAM_Veg), and reproductive SAM (SAM_Rep). Numbers above the heatmap show the number of samples: four, eight, and eight RNA-seq data sets were analyzed for mature leaf blade, vegetative SAM, and reproductive SAM, respectively. c Fraction of smRNAs for each size class relative to total smRNAs expressed in mature leaf blade (left, Leaf), vegetative SAM (middle, SAM_Veg), and reproductive SAM (right, SAM_Rep). The smRNAs were divided into nine classes according to their size. d Exponentially modified protein abundance index (emPAI)41 values for OsAGO4a (dark gray) and OsAGO4b (light gray) from proteomic analysis. emPAI is quantitative information proportional to the protein concentration in the samples. ND not detected. #1 and #2 indicate each replicate of the proteomic analysis. e Density plots show the frequency distribution of CHH methylation differences within 50-bp windows of the Rep hyper CHH DMRs (left), Leaf hypo CHH DMRs (middle), and Leaf hyper CHH DMRs (right) between osdrm2 knockout and WT leaf.
