Fig. 2: Loss of TRESK affects adaptive behavioural responses to light.
From: TRESK is a key regulator of nocturnal suprachiasmatic nucleus dynamics and light adaptive responses
a Bioluminescence recording of Per2::Luc WT and TRESK−/− SCN slices shows maintained clock gene rhythmicity even in the absence of TRESK. Grouped and averaged over three animals for WT and three for TRESK−/−. b 24-H locomotor activity profile of animals housed under 400 lux illumination, resulting in similar locomotor activity pattern in both genotypes, although significantly increased activity during the day in TRESK−/−. c Representative actograms of WT and TRESK−/− mice housed at 400 lux with 12:12 LD cycle and constant dark (DD). Light regime is indicated at the top of actograms, where yellow bars represent lights on and grey bars lights off. d 24-H free-running locomotor activity profile with TRESK−/− mice displaying similar activity pattern as the WT. e 24-H infrared beam breaks during different illumination housing and free-running, showing light-dependant behaviour with greatest differences at 2000 lux. Housing in constant dark for 4 weeks eliminated light-induced behavioural differences. Two-way ANOVA for housing conditions (F(3, 59) = 18.8, p < 0.0001) and genotype (F(3, 59) = 20.2, p < 0.0001), Bonferroni’s multiple comparisons test for LD 2000 lux p < 0.0001, DD 1 week p = 0.036. All grouped data are mean ± SEM; shaded areas in b and d show SEM. *p < 0.05, ****p < 0.0001. Source data are provided as a Source Data file.
