Fig. 1: Detection of EBOV. | Nature Communications

Fig. 1: Detection of EBOV.

From: Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

Fig. 1

a Schematic of the SHERLOCK EBOV assay. b, c Detection of a serial dilution of EBOV synthetic DNA using (b) mean fluorescence of three technical replicates and (c) lateral flow readouts. Error bars indicate ±1 SD for three technical replicates. d Test of cross-reactivity using MARV, EBOV, and LASV viral seedstock cDNA. Heat map is measured in Fluorescence (a.u.). e SHERLOCK testing of cDNA extracted from 12 confirmed EBOV-positive and 4 confirmed EBOV-negative samples collected from suspected EVD patients during the 2014 outbreak in Sierra Leone. Error bars indicate 95% confidence interval. f Four of the samples from e were also tested by collaborators using lateral flow detection. g, h Detection of serial dilution of synthetic RNA from Ituri, DRC and Makona, Sierra Leone using (g) fluorescence where error bars indicate ±1 SD for three technical replicates and (h) lateral flow readouts carried out at USAMRIID. Source data are in the Source Data file.

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