Fig. 2: Phenotypic clustering and in vivo expression profiling of phosphatases in C. neoformans.

In vitro phenotypic traits were examined under 30 different growth conditions and scored on a 7-point scale (−3: strongly reduced/susceptible, −2: moderately reduced/susceptible, −1: weakly reduced/susceptible, 0: wild-type like, +1 weakly enhanced/tolerant, +2: moderately enhanced/tolerant, +3: strongly enhanced/tolerant). All phenotypic data are available in the Cryptococcus neoformans Phosphatase Phenome Database (http://phosphatase.cryptococcus.org). More than three biologically independent experiments were performed for each phenotypic trait. Hierarchical phenotypic clustering of 60 phosphatases showing at least one phenotypic trait was performed with one minus Pearson correlation in Morpheus (https://software.broadinstitute.org/morpheus). The right panel shows the corresponding in vivo gene expression profiles for each phosphatase gene determined by NanoString nCounter platform analysis during intranasal murine infection with C. neoformans. Red letters represent pathogenicity-related phosphatases. The sdp101Δ mutant, which did not show any in vitro phenotypes but exhibited reduced infectivity, was also included. Abbreviations: 25 25 °C, 30 30 °C, 37 37 °C, 39 39 °C, CAP capsule production, MEL melanin production, URE urease production, MAT mating, HPX hydrogen peroxide, TBH tert-butyl hydroperoxide, MD menadione, DIA diamide, MMS methyl methanesulphonate, HU hydroxyurea, 5FC 5-flucytosine, AMB amphotericin B, FCZ fluconazole, FDX fludioxonil, TM tunicamycin, DTT dithiothreitol, CDS cadmium sulfate, SDS sodium dodecyl sulfate, CR Congo red, CFW calcofluor white, KCR YPD + 1.5 M KCl, NCR YPD + 1.5 M NaCl, SBR YPD + 2 M sorbitol, KCS YP + 1 M KCl, NCS YP + 1 M NaCl, SBS YP + 2 M sorbitol.