Fig. 5: The role of retromer complex in the pathogenicity of C. neoformans.
From: Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans
a Phenotypic heatmap of retromer complex mutants constructed based on in vitro phenotypic traits [vps29∆ (YSB4881, YSB4882), vps26∆ (YSB5671, YSB5672), vps35∆ (YSB5615, YSB5616), vps5∆ (YSB5683, YSB5684), and vps17∆ (YSB5724)] (Supplementary Fig. 12). b, c The retromer complex is involved in the pathogenicity of C. neoformans. b Insect-killing assay performed for the retromer complex mutants (n ≥ 15). P values shown in the graph were calculated using the log-rank (Mantel–Cox) test to measure statistical differences between the wild-type (WT) strain (H99S) and each phosphatase mutant strain. In the VPS26 graph, filled and empty circles indicate two independent PBS and WT-infection controls. c Signature-tagged mutagenesis (STM)-based murine infectivity assay (n = 3). STM scores were calculated by quantitative PCR. The ste50∆ and ire∆ mutants were used as virulent-positive control and avirulent-negative control strains, respectively. The statistically significant was calculated by one-way ANOVA analysis with Bonferroni’s multiple comparison test. Data are presented as mean values ± SEM (*P < 0.05).
