Fig. 6: O-linked glycan profiles of C. neoformans gda1Δ and ynd1Δ strains.
From: Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans
a HPLC profiles of O-linked glycans assembled on the cell wall mannoproteins of C. neoformans wild-type (WT), gda1Δ (YSB4750), and ynd1Δ (YSB4856) strains. To distinguish small peaks more clearly, HPLC profiles from 22 to 32 min in the x- and y-axes were enlarged and inserted. diamond, unknown peak. M mannose, X xylose, M3* α1,2-mannotriose. Arrow, decreased M3 and M4 peak; triangle, increased O-glycan species containing xylose (X1M2–X1M4) in the mutant strains. b O-glycan composition of each C. neoformans strain. For quantitative analysis, the area of each peak in the HPLC profile from three independent experiments was measured and presented as percentage of the total area of all peaks. Statistical significance was calculated by one-way ANOVA analysis with Bonferroni’s multiple comparison test. Data are presented as mean values ± standard deviation (SD) (*P < 0.05; **P < 0.001; ***P < 0.0001). c The proposed function of Gda1p and Ynd1p to provide donor substrates for mannosylation of proteins and lipids in the Golgi apparatus of C. neoformans.
