Fig. 2: KIN10 plays crucial roles in sugar-induced stomatal development.
From: KIN10 promotes stomatal development through stabilization of the SPEECHLESS transcription factor
a–c Quantification of the stomatal index of wild-type plants, KIN10-Ox and kin10 mutants. Seedlings of wild-type plants (Ler and Col-0), p35S::KIN10-HA (Ler background), p35S::KIN10-myc (Col-0 background), kin10 mutant and kin11 were grown in ½ MS liquid medium with or without 1% sucrose for 10 days under long-day condition. Error bars indicate standard deviation (S.D.) (n = 15–20). Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher’s LSD multiple comparisons test, p < 0.05). d–f Overexpression of KIN10 increased the percentage of SPCH-YFP labeled cells to total epidermal cells. Seedlings of pSPCH::SPCH-GFP and pSPCH::SPCH-GFP/KIN10-Ox (p35S::KIN10-myc-2#) were grown in ½ MS liquid medium with 1% sucrose concentration conditions for 3 days under 16 h light/8 h dark photoperiod. SPCH-GFP is in green, PI-marked cell outlines are in purple. Scale bars in confocal images represent 20 μm. Error bars indicate S.D. (n = 20). Asterisk between bars indicated statistically significant differences between the samples (Student t test, ****p < 0.0001).
