Fig. 1: ITGB3 is required for EV-induced colony formation in MDA.MB.231 cells. | Nature Communications

Fig. 1: ITGB3 is required for EV-induced colony formation in MDA.MB.231 cells.

From: ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells

Fig. 1

a Representative pictures are shown for each cell population and condition. b Conditioned medium (CM) was collected from exponentially growing MDA.MB.231 shCON, shITGB3, or IMR90 cells. In the case of vesicle-depleted CM (CM without exosomes), the same CM was split into two parts: one was used directly and the other was depleted of vesicles by ultracentrifugation before use, to establish the clonal cell growth response to the CM. Data are normalized to the non-conditioned control medium (DMEM). c, d Flow cytometry analysis for MDA.MB.231 shCON, MDA.MB.231 shITGB3 cells and IMR90 cells after incubating with 2–5 µg mL−1 fluorescently labelled EVs derived from both MDA.MB.231 shCON and MDA.MB.231 shITGB3 cells at different time points. Source data are provided as a Source Data file.(*p value <0.05, **p value <0.01, ***p value <0.001, ****p value <0.0001; n = 7 for non-CM condition; n = 4 for CM shCON, CM shITGB3, and CM IMR90; n = 3 for CM wo EVs shCON and CM wo EVs IMR90. n = 3 for panel 1c, d). Data are represented as mean ± SD in (b–d). Statistical analysis including two-way ANOVA multiple comparisons was carried out using GraphPad Prism 6.01.

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