Fig. 3: Interaction of EVs with the cell surface is a highly dynamic process. | Nature Communications

Fig. 3: Interaction of EVs with the cell surface is a highly dynamic process.

From: ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells

Fig. 3

Internalization of 2–5 µg mL−1 PKH26-labelled EVs derived from MDA.MB.231 cells by MDA.MB.231 shCON or MDA.MB.231 shITGB3 cells, measured by FACS. a Uptake of vesicles was measured by FACS after subjecting the cells to acid washing. b Internalization of 2–5 µg mL−1 PKH26-labelled EVs derived from MDA.MB.231 cells by MDA.MB.231 WT cells, pretreated with 20 μM Dyngo4a and pre-treated with 10 μg mL−1 heparin, with or without subjecting the cells to acid washing. c Representative confocal pictures used for 3D reconstruction. d Representative 3D reconstruction picture of MDA.MB.231 shCON cells. Bar represents 5 µm. e Analysis of EV internalization in MDA.MB.231 shCON, shITGB3 and shDyn2 cells after 3D reconstruction. Source data are provided as a Source Data file. (***p value < 0.001, ****p value < 0.0001, n = 3). Data are represented as mean ± SD in (a, b, e). Statistical analysis including two-way ANOVA multiple comparisons was carried out using GraphPad Prism 6.01.

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