Fig. 4: Characterization of EVs isolated from shCON and shITGB3 cells. | Nature Communications

Fig. 4: Characterization of EVs isolated from shCON and shITGB3 cells.

From: ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells

Fig. 4

a Schematic workflow for the preparation of EV samples for LC–MS/MS analysis. b Vesicle size distribution based on NTA data. c Particle concentration of isolated vesicle fractions normalized per million cells. d Representative CryoEM images of EV isolated from cultured MDA.MB.231 shCON and MDA.MB.231 shITGB3 cells. Source data are provided as a Source Data file. Scheme created with BioRender (https://biorender.com/). (*p value < 0.05, n = 3). Data are represented as mean ± SD in (b, c). Statistical analysis including two-tailed unpaired Student’s t test data was carried out using GraphPad Prism 6.01.

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