Fig. 5: Alterations in the proteome of shITGB3-derived EVs. | Nature Communications

Fig. 5: Alterations in the proteome of shITGB3-derived EVs.

From: ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells

Fig. 5

a Heat map representation of the normalized protein abundance (Log TOP 3 area) (q.value < 0.05) for the comparison of experimental replicates. b Scatter plot comparison of the abundance (Log TOP3 area) of proteins in the LC–MS/MS analysis of MDA.MB.231 shCON− and MDA.MB.231 shITGB3-derived EVs. The proteins validated by Western blot are highlighted. c Representative immunoblot analysis of candidate proteins identified in the proteomic survey. d Representative immunoblots after separation of vesicles in an iodixanol gradient for both MDA.MB.231 shCON (upper panel) and MDA.MB.231 shITGB3 (lower panel). e Representative immunoblot analysis of input and fractions 6 and 7. Source data are provided as a Source Data file.

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