Fig. 2: HMGB1 is increased in ALI and accumulates in aorta. | Nature Communications

Fig. 2: HMGB1 is increased in ALI and accumulates in aorta.

From: Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages

Fig. 2

(a) Western Blot (WB) analysis of HMGB1 from lungs of control or ALI mice (n = 3 per group) and quantification of bands normalized to β-actin. AU, arbitrary units. *P = 0.0493. (b, c) Immunofluorescence (IF) staining of HMGB1 in lungs of mice with conditions as indicated (scale bar = 20 µm). (d) Enzyme-linked immunosorbent assay (ELISA) of HMGB1 in serum of control or ALI mice (n = 8 per group). *P = 0.0176. (e) IF staining of HMGB1 in aortas of mice as indicated and quantification of staining (scale bar = 200 µm). Dotted lines outline the adventitial region. L, lumen; A, adventitia. n = 3 per group. AU, arbitrary units. ***P = 0.0003. (f) WB of HMGB1 in aortas of mice treated as shown and quantification of bands. n = 3 per group. AU, arbitrary units. **P = 0.0061. g Representative H&E and IF images of HMGB1 in healthy and AAA aortic human tissue (scale bar H&E = 500 µm, IF = 10 µm). L, lumen; A, adventitia. Inset indicate areas of IF images. (h) Quantitative-RT-PCR (qRT-PCR) analysis of Tlr4, Tlr2 and Ager mRNA in aorta of mice treated with PBS (Control) or Ang II. n = 3 per group for Tlr4, n = 4 per group for Tlr2, n = 4 (Control) and 3 (Ang II) for Ager. *P = 0.0171. (i) IF staining and quantification of TLR4 in aortic sections as indicated (scale bar = 10 µm). AU, arbitrary units. n = 3 per group *P = 0.036. Data is presented as mean, error bars represent s.e.m. P values were calculated using two-tailed unpaired t-tests (a, d, f–i) or one-way ANOVA (e).

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