Fig. 2: 1D 1H NMR spectra of microgram amount of strychnine in gels. | Nature Communications

Fig. 2: 1D 1H NMR spectra of microgram amount of strychnine in gels.

From: Relative configuration of micrograms of natural compounds using proton residual chemical shift anisotropy

Fig. 2

a 1D 1H NMR spectrum of 300 μg strychnine in protonated PMMA gel. The spectrum was acquired with 256 scans. Note that only few peaks from the analyte are visible (indicated by the blue arrows) and many signals are masked by the polymer signals. b 1D 1H NMR spectrum of 80-μg strychnine in deuterated PMMA gel. The spectrum was acquired with 800 scans. Blue arrows on the inset in (b) highlight some strychnine resonances that become visible due to the removal of proton signals of the polymer. Both spectra were recorded in a Bruker 800 MHz NMR spectrometer equipped with a TCI cryoprobe. Stretching devices with an inner diameter of 4.0 mm for minimum and 3.2 mm for maximum alignment were used. c 1D 1H NMR spectrum of 10-μg strychnine acquired with 8192 scans in deuterated PMMA gel under maximum alignment condition. For clarity, expansions for some of the proton signals (H4, H22, H8, and H20a) are also shown.

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