Fig. 2: Viral RNA (vRNA) abundances in A/Panama-infected A549 cells 10 h.p.i.

a Relative abundances of all vRNA segments per cell as measured by fluorescence in situ hybridisation (FISH). For each of the measured cells (n = 69 from four independent experiments), the number of spots that were detected was counted and normalised to the total number of spots per cell. The colouring scheme is the same as in Fig. 1. b Data shown in a in a Tukey boxplot representation. Upper bound of box, centre and lower bound of the box represent the 75th percentile, the 50th percentile (median) and the 25th percentile, respectively. Upper and lower whiskers represent the maxima and minima of the boxplots showing the respective largest or smallest value within 1.5× interquartile range above the 75th or below the 25th percentile (bounds of box). In all, 12.5% (red line) corresponds to the expected fraction for each vRNA in a uniform distribution. For the segments, there is no significant difference (ANOVA, F test, one-sided, α = 0.05) between the cells (P > 0.999). The application of ANOVA requires Gaussian distribution (fulfilled, see Supplementary Fig. 7) and the same variability (standard deviation) for the segments. However, the Bartlett test (one-sided, α = 0.05) shows that the variability of the individual segments is significantly different (P > 0.0001). The Kruskal–Wallis test does not presuppose homogeneity of variability, but agrees with the F test in the result, i.e., no significant differences (one-sided, P > 0.999). Source data are provided as a Source Data file.