Fig. 3: Distribution of MSC ranks in A/Panama-infected A549 cells after 10 h.p.i.
From: Selective flexible packaging pathways of the segmented genome of influenza A virus
A total of 3.9 × 105 distinct segment spots were detected in A/Panama-infected A549 cells and binned into about 1 × 105 MSCs. The frequency distributions of MSC ranks (a–d, ‘Rank Distributions’, filled bars: ‘MSC counts’, empty bars: ‘Segment counts’), of all segments S1–S8 occurring in a cell (e–h, ‘Total Segments’) and of free, i.e., monomeric segments, i.e., rank 1 (i–l, ‘Free Segments’) are shown. Frequency distributions for all 69 cells (a, e, i, ‘All Cells’) and exemplary for a single cell (e.g., ‘Cell 18’ b, f, j). Frequency distributions for cells in which particular segments appeared in unusually low quantities: counts were low for S1 and S8 in ‘Cell 23’ c, g, k, and for S8 in ‘Cell 29’ (d–l). In a, values are shown as mean ± s.e.m. Statistics for ‘Total Segments’: a multiple comparison of means by Tukey test (one-sided, α = 0.05) reveals the following significant differences between segments: a S2–S8 and S5–S8 (P = 0.041; *P ≤ 0.01); Source data are provided as a Source Data file.
