Fig. 6: Viral RNA (vRNA) and MSC rank abundances in A/Mallard-infected A549 cells 10 h.p.i.

a Relative abundances of all vRNA segments per cell as measured by fluorescence in situ hybridisation (FISH). For each of the measured cells (n = 54 from three independent experiments), the number of spots that were detected was counted and normalised to the total number of spots per cell. The colouring scheme is the same as in Fig. 1. b Data shown in a in a Tukey boxplot representation. Upper bound of box, centre and lower bound of box represent the 75th percentile, the 50th percentile (median) and the 25th percentile, respectively. Upper and lower whiskers represent the maxima and minima of the boxplots showing the respective largest or smallest value within 1.5× interquartile range above the 75th or below the 25th percentile (bounds of box). In all, 12.5% (red line) corresponds to the expected fraction for each vRNA in a uniform distribution. For the segments, no significant difference (ANOVA, F test, one-sided, α = 0.05) between the cells was found (P > 0.999). The Bartlett test (one-sided, α = 0.05) shows that the variability of the individual segments is significantly different (P > 0.0001) (see also legend to Fig. 3). The Kruskal–Wallis test does not presuppose homogeneity of variability, but agrees with the F test in the result, i.e., no significant differences (one-sided, P > 0.999). Source data are provided as a Source Data file. c–e A total of 2 × 10⁵ distinct segment spots were detected in A/Mallard-infected A549 cells and binned into about 9 × 10⁴ MSCs. The frequency distributions of MSC ranks (c (filled bars: ‘MSC counts’, empty bars: ‘Segment counts’)), of all segments S1–S8 occurring in cells d and of free segments, i.e., rank 1, e are shown. Values are shown as mean ± s.e.m. Multiple comparison of means by Tukey test (one-sided, α = 0.05) reveals the following significant differences between segments d: S2–S4, S2–S5, S2–S8, S3–S8, S4–S7, S5–S7 and S7–S8 (P between 0.0001 and 0.0137; *P ≤ 0.01; **P ≤ 0.001; ***P ≤ 0.0001). Source data are provided as a Source Data file.