Fig. 6: Inhibition of Fyn attenuates heroin self-administration and cue-induced responding. | Nature Communications

Fig. 6: Inhibition of Fyn attenuates heroin self-administration and cue-induced responding.

From: Chromatin accessibility mapping of the striatum identifies tyrosine kinase FYN as a therapeutic target for heroin use disorder

Fig. 6

a Active lever presses during 3 h long heroin self-administration session in vehicle and saracatinib treated Long-Evans rats. Arrows represent days of saracatinib administration. Two-way ANOVA revealed significant group effect (n = 6/group, F1,29 = 6.852, p = 0.0139) indicating decreased heroin self-administration behavior on days of saracatinib exposure. b Inactive lever presses were not affected in the same sessions (n = 6/group, two-way ANOVA, F1,29 = 0.096, p = 0.7589). c Active lever presses during a 1 h long, cue-induced, non-reinforced session in rats previously trained to self-administer heroin that were injected with vehicle or saracatinib (n = 6/7 for vehicle/saracatinib, two-way ANOVA, F1,132 = 7.376, p = 0.0075). Saracatinib significantly decreased responding for the heroin-paired stimulus. d Inactive lever presses were not affected in the same sessions (n = 6/7 for vehicle/saracatinib, two-way ANOVA, F1,132 = 3.165, p = 0.0775). e Active lever presses during a 1 h long, cue-induced, non-reinforced session in rats previously trained to self-administer heroin and subjected to siRNA-mediated knock-down (KD) of Fyn in the dorsal striatum 3 days prior to the session (n = 5/group, two-way ANOVA, F1,96 = 4.007, p = 0.0481). KD of Fyn significantly decreased responding to the heroin-paired cue. f Inactive lever presses were not affected in the same sessions (n = 5/group, two-way ANOVA, F1,96 = 0.7685, p = 0.3828). g Active lever presses during a 1 h long, drug-induced, non-reinforced session in rats previously trained to self-administer heroin and subjected to siRNA-mediated knock-down of Fyn in the dorsal striatum 3 days prior to the session (n = 6/group, two-way ANOVA, F1,120 = 8.545, p = 0.0041). KD of Fyn significantly decreased active lever pressing. h Inactive lever presses were not affected in the same sessions (n = 6/group, two-way ANOVA, F1,120 = 0.6884, p = 0.4083). Data are represented as mean ± SEM. Source data are provided as a Source Data file.

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