Fig. 5: Bioreactor system with continuous extraction.
From: Isobutanol production freed from biological limits using synthetic biochemistry
a Bioreactor set up for continuous product extraction. Phenetole in continuously added from a reservoir and simultaneously extracted at the same rate. The extracted layer is collected in a separate container. b Isobutanol titer over time using the continuous extraction system. c Residual activity of enzymes after 70 hours of incubation. d Isobutanol titer over time with the addition of a second bolus of KivD-S and BsAlsS-P at 33 h, the enzymes that become inactivated after 70 h. The ATP (green) and NADPH (blue) concentrations in the reaction are also shown as a function of time. Error bars in all panels are the standard deviation of technical triplicates. Open circles in panel c show the data points. Source data underlying Fig. 5b–d are provided as a Source data file.
