Fig. 1: Loss of TET2 leads to dysregulated lobuloalveolar development and impaired luminal lineage commitment.
From: TET2 directs mammary luminal cell differentiation and endocrine response
a Immunoblot showing TET2 protein expression in WT, HET, and KO mammary tissues. b, c Whole mount, H&E, and Masson’s Trichrome staining of mammary tissues from 7-week-old WT, HET, and KO virgin female mice. Arrow indicating light blue staining of collagen enriched fibrosis regions (scale bar: 50 μm). d, e H&E staining of mammary tissues from WT, HET, and KO pregnant mice (day 18.5, scale bar: 50 μm) and lactating mice (day 10, scale bar: 200 μm). Arrows indicating lipid droplets in pregnant mouse tissues (d) and milk in lactating mouse tissues (e), respectively. f Immunoblots showing expression of β-casein and estrogen receptor-α (ERα). g Flow cytometry analysis showing the percentage of the basal MaSC-enriched cell population (MaSCe, Lin−CD24+CD29hi, indicated by a red circle) and luminal cell population (Lum, Lin−CD24+CD29lo, indicated by a green circle), and h the percentage of basal MaSC-enriched cell population (MaSCe, Lin−CD24+CD29hiCD61hi, indicated by a red circle), luminal progenitor cell population (LP, Lin−CD24+CD29loCD61hi, indicated by a black circle), and mature luminal cell population (ML, Lin−CD24+CD29loCD61lo, indicated by a green circle) isolated from n = 3 biologically independent mammary epithelial cell samples of 7-week-old- WT, HET, and KO female mice (n = 3 animals/group). i Representative image and the number of primary mammospheres (scale bar: 50 μm) generated from WT, HET, and KO mammary epithelial cells per 1000 seeding cells (n = 3 independent experiments), and j the number of the serially passaged spheres generated from WT and KO cells (n = 3 independent experiments). Data were presented as mean ± SD. p-values were determined by two-sided Student’s t-test; asterisk indicates p < 0.05, double asterisks indicate p < 0.01 (compared to WT). k, l Representative confocal immunofluorescence images showing co-staining of CK8 with CK14 or MUC1 with SMA in mammary glands from 7-week-old- WT and KO female mice (scale bar: 50 μm). Source data are provided as a source data file.
