Fig. 3: In vivo validation of subclusters and markers in the lymph gland.
From: Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila
a Expression of GST-rich-marker genes, CG18547 and CG3397. mRNA transcripts of CG18547 (top; magenta) or CG3397 (bottom; magenta) are visualized at the outer demarcation of Tep4 (green; left). Magnified images: Tep4 and CG18547 or CG3397. b Expression of adipohemocyte-specific marker genes, Sirup or Lsd-2. A few Sirup-expressing (top; magenta) or Lsd-2 (bottom; magenta)-expressing cells are observed within the Hml+ (green) cortical zone. However, Sirup-positive or Lsd-2-positive hemocytes are devoid of Hml. Magnified images: Hml and Sirup or Lsd-2. c Lipid droplet-containing hemocytes in the lymph gland. When visualized with BODIPY (top; green) or Nile Red (bottom; magenta) lipid probes, a few hemocytes (Phalloidin, white) at the cortical zone hold a lipid droplet. Magnified images: actin structures (Phalloidin, white), a lipid droplet, and DAPI. d Plasmatocyte marker, vir-1 (green; vir-1MiMiC) partially co-localizes with Pxn+ (top; magenta) or NimC1 (bottom; magenta). Magnified images: vir-1 and Pxn or NimC1. e PSC markers in the lymph gland. Antp+ PSC cells (magenta) co-localize with Ilp6 (green; Ilp6-gal4 UAS-mCD8::GFP), tau (green; tauMiMiC), mthl7 (white; mthl7-gal4 UAS-GTRACE), or chrb (green; chrbMiMiC). Ilp6-gal4 is expressed in other cell types including PH6 and crystal cells (Supplementary Fig. 2i). Magnified images: Antp and Ilp6, tau, mthl7, or chrb. f Crystal cell markers in the lymph gland. lz+ crystal cells (magenta) co-localized with Men (white; Men-gal4 UAS-GTRACE; left) or Numb (green; Numb::GFP; right). lz co-localizes with a subset of Men while all the Numb+ and lz+ overlap. Magnified images: lz and Men or Numb. g domeless-LexA generated in this study (magenta; domeless-LexA LexAop-mRFP-nls) partially co-localizes with Pxn (green). Magnified images: domeless and Pxn. White scale bar, 30 μm; yellow scale bar, 3 μm. Lymph gland is demarcated by white dotted line. Blue indicates DAPI. The dotted box in a–g indicates a magnified region. Each panel at the right displays magnified views.
